NEW YORK, Feb. 20, 2018 (GLOBE NEWSWIRE) --
Global Competent Cells Market by Type (Chemically Competent Cells, Electro competent Cells) by Application/End User (Subcloning & Routine Cloning, Phage Display Library Construction, Toxic/Unstable DNA Cloning, High-Throughput Cloning, Protein Expression, Mutagenesis, Single-Stranded DNA Production, Bacmid creation, Cre-lox recombination (PIR1/PIR2)) by Competence (Natural Competence, Artificial Competence) Industry Analysis, Volume, Share, Growth, Challenges, Trends, and Forecast 2016–2024.
Competent cells incorporate foreign DNA through altered cell walls to pass DNA more easily. Calcium chloride and Heat shock treatment are used to make cell “competent”. Electroporation is a process that includes brief exposure of cells to an electric field in order to pass DNA through it. Cells undergoing very rapid growth can be made competent easily than cells in other development phase. Bacterial culture has varying growth rate, in initial hours called lag phase with small number of dividing cells growth rate is low. In rapid growth phase also called Log phase under proper temperature cells can become competent easily.
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Competent Cells Market: Competence Type
Competence is distinguished based on the environment used to perform on bacterial cell transformation. Basically Competence type are:
Natural Competence
Cells are able to receive DNA from their environment by:
• Conjugation
• transformation
• transduction
In transformation the DNA is directly pass into the cell. In order to receive transforming DNA recipient cells should be in a specialized physiological state called log phase or competent state.
Artificial Competence
Artificial competence includes setting specific artificial conditions in which cells are made permeable to DNA in laboratory. It is relatively easy competence procedure and can be used in the genetic engineering of bacteria. Artificial competence is low efficiency process as cells are forced to adopt foreign DNA.
Major Market Players:
• Thermo Fisher Scientific
• Agilent Technologies
• Takara Bio
• Promega Corporation
• Beijing TransGen Biotech
• GeneScript Corporation
• Yeastern Biotech
• New England Biolabs
• QIAGEN N.V.
• OriGene Technologies
• Lucigen
• Zymo Research
• Bio-Rad Laboratories
• Bioline
• Delphi Genetics
• IBA GmBH
• Cell Applications
• BioDynamics Laboratory
• Scarab Genomics
• GCC Biotech
• SMOBIO Technology
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